Laboratory Services

Varicella Zoster Qualitative by PCR, Tissue or Cells

Name: Incyte Diagnostics
Address: 13103 E Mansfield Ave, Spokane Valley, Washington, 99216, USA
Telephone: +1-If-you-have-questions-regarding-your-bill-or-would-like-to-make-a-payment,-please-visit-our-Billing-page-by-clicking-"Pay-Online"-at-the-top-of-the-page-For-other-inquiries,-please-call-us-at-509-892-2700

Updated Test Information:

Test Description	Varicella Zoster Qualitative by PCR, Tissue or Cells
Synonym(s)	VZ by PCR-Tissue or Cells, V Zoster Virus by PCR-Tissue or Cells, UOW518
Test ID	VZVQLT
General Information	The detection of Varicella Zoster Virus (VZV) by real-time PCR amplification. The primers used in this PCR reaction amplify a fragment of the ORF62 region. Detection of VZV DNA (copies/mL) is achieved by amplifying a standard curve in each run, consisting of serial dilutions of a known amount of DNA containing the VZV amplicons. The minimum virus level that gives a positive result in 95% of replicates is 75 copies/mL (1.88 log copies/mL). Results are reported in copies per milliliter (copies/mL). This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
Container Type	Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM). Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube. Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube. No Heparin aspirate in syringe. Cellular fluid: 1 mL in sterile tube.
Specimen Type	Tissue Biopsy, Paraffin Tissue, Bone Marrow or Cellular fluid (e.g. BAL or Washing)
Specimen Requirements	Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.
Specimen Collection / Processing Instructions	Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw. Paraffin Tissue: Ship at room temperature. Bone Marrow: Ship at room temperature within 24 hours of collection. Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Minimum Sample Volume	0.5 mL
Unacceptable Specimen Conditions	nacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.
Department (code)	Virology, Eastlake
Methodology	Real-time Polymerase Chain Reaction
Testing Schedule	Extraction Tuesday and Friday. PCR following Wednesday and Monday respectively.
Test Includes	VZVQLS VZV Qual.Specimen Descp. VZVQLR VZV Qual Result VZVQLI VZV Qual Interp
CPT Code(s)	87798
STAT Orderable (Y/N)	No
Performing Lab	UW
LOINC Code(s)	11483-5