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Pyridoxal 5-Phosphate (PLP), Plasma

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Updated Test Information:

Test Description
Pyridoxal 5-Phosphate (PLP), Plasma
Synonym(s)

B6 (Vitamin); PALP (Pyridoxal 5-Phosphate); Pyridoxal 5-Phosphate (PALP); Pyridoxal Phosphate; Vitamin B6
Test ID
PLP
General Information

Useful for:



  • Determining vitamin B6 status, including in persons who present with progressive nerve compression disorders, such as carpal tunnel and tarsal tunnel syndromes

  • Determining the overall success of a vitamin B6 supplementation program

  • Diagnosis and evaluation of hypophosphatasia
Specimen Type

Heparinized Plasma
Specimen Requirements

Green Top (sodium or lithium heparin)
Specimen Collection / Processing Instructions

  • Patient Preparation:

    • Fasting-overnight (12-14 hours) (infants-draw prior to next feeding)

    • Patient must not ingest vitamin supplements for 24 hours before the specimen is drawn

    • Centrifuge at 4 degrees C within 2 hours of collection, then aliquot all plasma into amber vial.
Minimum Sample Volume

0.5 mL
Additional Processing Details

Ship specimen in amber vial to protect from light
Stability

  • LIGHT PROTECTED CONTAINER

  • Frozen: 14 days 
Unacceptable Specimen Conditions

Refrigerated specimens
Limitations

Reference ranges were established using healthy fasting volunteers who abstained from vitamin supplementation for 24 hours prior to collection. Vitamin supplementation and nonfasting may result in elevated plasma vitamin concentrations
Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Estimated TAT

1-4 days
Testing Schedule

Monday-Friday
CPT Code(s)

84207
Reference Range

5-50 mcg/L
Performing Lab

Mayo
LOINC Code(s)

30552-4
Additional Information