Laboratory Services

Lactic Acid

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Updated Test Information:

Test Description
Lactic Acid
Synonym(s)

Lactic Acid

Test ID
LA
General Information

Anaerobic glycolysis markedly increases blood lactate, especially with prolonged exercise. The common cause for increased blood lactate is anoxia resulting from such conditions as shock, pneumonia and congestive heart failure. Lactic acidosis may also occur in renal failure and leukemia. Thiamine deficiency and diabetic ketoacidosis are associated with increased levels of lactate and pyruvate. Lactate measurements that evaluate the acid‑base status are used in the diagnosis and treatment of lactic acidosis (abnormally high acidity in the blood).

Specimen Type

Plasma

Alternate Specimen Type

Heparinized plasma is acceptable, but precautions must be taken to retard glycolysis by keeping the whole blood on ice and then separating the plasma from the cells within 15 minutes of collection.

Specimen Requirements

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Specimen Collection / Processing Instructions

Centrifuge and aliquot plasma to an aliquot tube within 15 minutes of collection. Avoid the use of a tourniquet, if possible

Minimum Sample Volume

1 mL

Pediatric Min. Volume (if applicable)

500 uL

Additional Processing Details

Centrifuge and aliquot plasma to an aliquot tube within 15 minutes of collection. Avoid the use of a tourniquet, if possible

Stability

Stability in plasma (separated):
8 hours at 15‑25 °C
14 days at 2‑8 °C

Stability in plasma (heparinized):
38 days at -20 °C

Unacceptable Specimen Conditions

Do not use serum specimens.

Limitations

The lactate level increases rapidly with physical exercise. The time required for return to normal lactate values depends on the physical fitness of the subject. 30 minutes at rest is usually sufficient for this purpose.
Blood samples should be drawn from a stasis‑free vein. However, minimal hemostasis (less than 30 seconds) will not affect lactate levels. Avoid the use of a tourniquet, if possible.
Glycolysis in blood samples can rapidly increase lactate levels. Cells contribute to the glycolysis and their quick removal is essential for accurate lactate analysis.

Methodology

Colorimetric

Estimated TAT

0-2 days

Testing Schedule

Monday-Saturday

CPT Code(s)

83605

Reference Range

4.5-19.8 mg/dL

Performing Lab

Incyte Diagnostics

LOINC Code(s)

2524-7