Laboratory Services
Hepatitis C RNA, Quantitative
Print this pageUpdated Test Information:
| Test Description |
Hepatitis C RNA, Quantitative
|
|
|---|---|---|
| Synonym(s) |
HCV RNA Quant, Hepatitis C by PCR, PCR-Hepatitis C, Viral Load-Hepatitis C, UOW181 |
|
| Test ID |
HCVQNT
|
|
| General Information |
The Abbott real-time FDA approved HCV assay detects and quantifies Hepatitis C virus by real-time PCR amplification. The Abbott assay utilizes three master mixes, with each mix containing three primer/probe sets, with 1a and 1b subtyping in the NS5b region, and the remaining primer sets are in the 5’UTR region. The RNA concentration detected with a probability of 95% or greater is 12 IU/mL (1.08 log IU/mL). |
|
| Container Type |
Blood: 5 mL blood in PEARL TOP tube (PPT). Also Acceptable: 5 mL blood in GOLD SST, RED, or LAVENDAR (EDTA) tube. |
|
| Specimen Type |
Serum |
|
| Specimen Collection / Processing Instructions |
Separate plasma/serum from whole blood within 6 hours of collection. |
|
| Minimum Sample Volume |
1 mL |
|
| Stability |
Freeze plasma/serum at -10°C or colder and ship on dry ice. Plasma may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Do not freeze/thaw. |
|
| Unacceptable Specimen Conditions |
Plasma collected in sodium or lithium heparin. |
|
| Department (code) |
Virology |
|
| Methodology |
Real-time Polymerase Chain Reaction |
|
| Testing Schedule |
Tuesday, Thursday (volume dependent), Saturday |
|
| Test Includes |
HCQNR Hepatitis C Quant Result |
|
| CPT Code(s) |
87522 |
|
| STAT Orderable (Y/N) |
No |
|
| Performing Lab |
UW |
|
| LOINC Code(s) |
50023-1 |
