Laboratory Services

Eye Fluid Viral PCR Quant Panel

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Updated Test Information:

Test Description
Eye Fluid Viral PCR Quant Panel
Synonym(s)

UOW3550

Test ID
EYEVQP
General Information

The detection and quantitation of CMV, Herpes Simplex Virus (HSV) and Varicella Zoster Virus (VZV) by real- time PCR from eye fluid.


The detection and quantitation of CMV by real-time PCR. The PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Quantitation of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is 300 IU/mL (2.48 log IU/mL).


The detection and quantitation of Herpes Simplex virus (HSV) by real-time PCR amplification. PCR detects amplicons of the gB gene for both HSV-1 and HSV-2. The two probes used in the reaction, specific either to HSV-1 or HSV-2 are labeled with different reporter dyes. Quantitation of HSV DNA (copies/mL) is achieved by amplifying a standard curve consisting of serial dilutions of a known amount of DNA containing the HSV-1 and HSV-2 amplicons. Results are reported in copies per milliliter (copies/mL). The minimum virus level that gives a positive result in 95% of replicates is 250 copies/mL (2.40 log copies/mL).


The detection and quantitation of Varicella Zoster Virus (VZV) by real-time PCR amplification. The primers used in this PCR reaction amplify a fragment of the ORF62 region. Quantitation of VZV DNA (copies/mL) is achieved by amplifying a standard curve in each run, consisting of serial dilutions of a known amount of DNA containing the VZV amplicons. The minimum virus level that gives a positive result in 95% of replicates is 75 copies/mL (1.88 log copies/mL). Results are reported in copies per milliliter (copies/mL).


This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

Container Type

Sterile container

Specimen Type

Eye Fluid

Specimen Requirements

Eye Fluid: 50 µL 

Specimen Collection / Processing Instructions

Do not send syringe with needle. Only send syringe or transfer fluid to sterile container.


Ship samples Frozen. Do not freeze/thaw.

Minimum Sample Volume

50 microliters

Methodology

Real-time Polymerase Chain Reaction.

Testing Schedule

Tuesday and Friday

Test Includes

Code                            Name
CMVEYE                  CMV Quant PCR for Eye Fluids
CMVQNS                 CMV Quant Specimen Description
CMQNR                   CMV Quant Result
CMLOG                    CMV DNA IU/mL (Log10)
CMVQNI                  CMV Quant Interpretation
HS12QN                  HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid
HSVQNS                  HSV Specimen_PCR Quant
HSVQN1                 HSV Type 1 Quant PCR Result
HSVLO1                  HSV1 DNA Copies/mL (Log 10)
HSVQN2                HSV Type 2 Quant PCR Result
HSVLO2                  HSV2 DNA Copies/mL (Log 10)
HSVQNI                  HSV Interpretation_PCR Quant
HSVQNM               HSV Type 1 and 2 Quant PCR Method
VZVEYE                  VZV Quant PCR for Eye Fluids
VZVQNS                 VZV Quant Specimen Descp.
VZVQNR                VZV Quant Result
VZVLOG                 VZV DNA Copies/mL (Log 10)
VZVQNI                   VZV Quant Interp.

CPT Code(s)

87497, 87530x2, 87799

STAT Orderable (Y/N)

No

Performing Lab

UW

LOINC Code(s)