Laboratory Services
CMV Quant PCR for Eye Fluids
Print this pageUpdated Test Information:
| Test Description |
CMV Quant PCR for Eye Fluids
|
|
|---|---|---|
| Synonym(s) |
CMV-Vitreous, UOW3052 |
|
| Test ID |
CMVEYE
|
|
| General Information |
The detection and quantitation of CMV by real-time PCR. The PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Quantitation of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is 300 IU/mL (2.48 log IU/mL). This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration. |
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| Container Type |
Sterile container |
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| Specimen Type |
Eye Fluid |
|
| Specimen Requirements |
50 microliters |
|
| Specimen Collection / Processing Instructions |
Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw. |
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| Minimum Sample Volume |
50 microliters |
|
| Stability |
Preferred Frozen |
|
| Department (code) |
Virology, Eastlake (EVIR) |
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| Methodology |
Real-time Polymerase Chain Reaction. |
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| Estimated TAT |
1 Day |
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| Testing Schedule |
Monday - Saturday |
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| Test Includes |
CMVQNS - CMV Quant Specimen Description |
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| CPT Code(s) |
87497 |
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| STAT Orderable (Y/N) |
No |
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| Performing Lab |
UW |
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| LOINC Code(s) |
