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Celiac Disease Panel

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Updated Test Information:

Test Description
Celiac Disease Panel
Synonym(s)

tTG, Tissue Transglutaminase, DGP, Deamidated Gliadin, Gliadin
Test ID
CELIAC
General Information

Celiac disease is a life-long condition in which ingestion of gluten leads to chronic inflammation and damage of the small intestinal mucosa. The detection of tissue transglutaminase (tTG) and deamidated gliadin (DGP) antibodies are highly disease specific serological markers for celiac disease and dermatitis herpetiformis. Patients with selective IgA deficiency may yield false negative tTG IgA and DGP IgA results. The use of tTG IgG and DGP IgG antibodies can be helpful markers in patients with this deficiency. Assay results should be used in conjunction with clinical findings and other serological tests. Antibodies directed against gliadin, its deamidated products, and tTG are dependent on the ingestion of gluten. For this reason, serology testing is best performed in patients on a gluten-containing diet. In children younger than 2 years, it is recommended to combine tissue transglutaminase (tTG) testing with deamidated gliadin (DGP) IgA and IgG.
Specimen Type

Serum
Alternate Specimen Type

Plasma (EDTA or Citrate)
Specimen Requirements

Tiger
Minimum Sample Volume

1.5 mL
Pediatric Min. Volume (if applicable)

750 uL
Additional Processing Details

Heparin interferes with the measurement of tTG antibodies.
Caution: Serum/plasma samples are not to be repeatedly frozen and thawed, since this can cause analyte deterioration. They are to be thawed only once.
Stability

Stored at 22˚C: 8 hrs
Stored at 2-8˚C: 1 week
If assays are not completed within 1 week or the specimen is to be stored beyond 1 week, the serum/plasma should be frozen at or below -20˚C.
Unacceptable Specimen Conditions

Li-Heparin, lipemic, hemolyzed or microbially contaminated samples.
Limitations

In children younger than 2 years, it is recommended to combine tissue transglutaminase (tTG) testing with deamidated gliadin (DGP) IgA and IgG.

Antibodies directed against gliadin, its deamidated products, and tTG are dependent on the ingestion of gluten. For this reason, serology testing is best performed in patients on a gluten-containing diet.
Methodology

Fluorescent enzyme immunoassay (FEIA)
Estimated TAT

2-5 days
Testing Schedule

Monday-Saturday, alternating days
Test Includes

Tissue Transglutaminase (tTG) IgA
Tissue Transglutaminase (tTG) IgG
Deamidated Gliadin IgA
Deamidated Gliadin IgG
CPT Code(s)

86258(x2)
86364(x2)
Reference Range
  Negative Equivocal Positive
Tissue Transglutaminase (tTG) AB, IgA <7 7-10 >10 U/ml
Tissue Transglutaminase (tTG) AB, IgG <7 7-10 >10 U/ml
Deamidated Gliadin AB, IgA <7 7-10 >10 U/ml
Deamidated Gliadin AB, IgG <7 7-10 >10 U/ml
Performing Lab

Incyte Diagnostics
LOINC Code(s)

46128-5, 56537-4