Test Directory

Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum

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Updated Test Information:

Test Description
Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum
Synonym(s)

ANA (Antinuclear Antibodies); Antinuclear Antibodies (ANA), HEp-2 substrate, Serum; LE (Lupus Erythematosus) Prep; Antinuclear Antibody, Serum; ANA IFA; ANA IF

Test ID
NAIFA
General Information

Useful for evaluating patients suspected of having systemic rheumatic disease

Specimen Type

Serum

Specimen Requirements

  • Serum Gel (SST)

  • Red Top

Stability

  • Refrigerated (preferred): 21 days

  • Frozen: 28 days

Limitations

Some patients without clinical evidence of an autoimmune disease or a systemic rheumatic disease may have a detectable level of antinuclear antibody (ANA). This finding is more common in women than men and the frequency of a detectable ANA in healthy women over 40 years of age may approach 15% to 20%. ANA may also be detectable following viral illnesses, in chronic infections, or in patients treated with many different medications.

Methodology

Indirect Immunofluorescence

Estimated TAT

1-2 days

Testing Schedule

Monday-Saturday

Test Includes

  • Antinuclear Ab, HEp-2 Substrate

  • ANA Titer

  • ANA Pattern

  • ANA Titer 2

  • ANA Pattern 2

  • Cytoplasmic Pattern

Retention

14 days

CPT Code(s)

86039

Additional Information
Minimum Sample Volume

0.3 mL

Reference Range

<1:80 (Negative)

Performing Lab

Mayo

LOINC Code(s)

59069-5, 33253-6, 49311-4, 33253-6, 49311-4, 55171-3, 77202-0