Laboratory Services
Anti Neutrophil Cytoplasmic Antibody
Print this pageUpdated Test Information:
| Test Description |
Anti Neutrophil Cytoplasmic Antibody
|
|
|---|---|---|
| Synonym(s) |
ANCA by IF, ANCA by Immunofluorescence, UOW691 |
|
| Test ID |
ANCAIF
|
|
| General Information |
Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis. |
|
| Container Type |
3 mL blood in RED TOP or SST tube |
|
| Specimen Type |
Serum |
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| Specimen Collection / Processing Instructions |
Refrigerate serum |
|
| Minimum Sample Volume |
0.5mL Serum |
|
| Limitations |
Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern. |
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| Department (code) |
Immunology |
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| Methodology |
Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies. |
|
| Testing Schedule |
Monday - Friday |
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| CPT Code(s) |
86036 |
|
| Reference Range |
Negative (NRN) Effective Date: 4/1/2010 |
|
| Performing Lab |
UW |
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| LOINC Code(s) |
