Laboratory Services

17-Hydroxypregnenolone, Serum

Updated Test Information:

Test Description

Test ID

17OHP

General Information

Useful for:

Ancillary test for congenital adrenal hyperplasia (CAH), particularly when 21-hydroxylase and 11-hydroxylase deficiency have been ruled out

Confirmation of 3-beta-hydroxy dehydrogenase (3-beta-HSD) deficiency

Evaluation of females with hirsutism or infertility

Specimen Type

Serum

Specimen Requirements

Preferred: Red top tube

Acceptable: Serum Gel

Minimum Sample Volume

0.5 mL

Stability

Frozen (preferred): 28 days

Refrigerated: 28 days

Limitations

At birth, the hypothalamic-pituitary-adrenal axis and the hypothalamic-pituitary gonadal axis are activated and adrenal and sex steroid levels are high. In preterm infants, the elevations can be even more pronounced due to illness and stress. As a result, preterm infants may occasionally have 17-hydroxypregnenolone levels of up to 9,799 ng/dL. Term infants (0-28 days) will have levels <3,104 ng/dL. These then fall over the following 2 years to prepubertal levels of <277 ng/dL.

Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Estimated TAT

2-6 days

Testing Schedule

Monday, Tuesday, Thursday

Retention

14 days

CPT Code(s)

84143

Reference Range

Age	Males: Range (ng/dL)	Females:Range (ng/dL)
Premature (26-28 weeks)	1219 - 9799	1219 - 9799
Premature (29-36 weeks)	346 - 8911	346 - 8911
Full term (1-5 months)	229 - 3104	229 - 3104
6 months-364 days	221 - 1981	221 - 1981
1-2 years	35 - 712	35 - 712
3-6 years	0 - 277	0 - 277
7-9 years	0 - 188	0 - 213
10-12 years	0 - 393	0 - 399
13-15 years	35 - 465	0 - 408
16-17 years	32 - 478	0 - 424
≥ 18 years	55 - 455	31 - 455
TANNER STAGES	Males: Range (ng/dL)	Females:Range (ng/dL)
Stage I	0 - 209	0 - 236
Stage II	0 - 356	0 - 368
Stage III	0 - 451	0 - 431
Stage IV - V	35 - 478	0 - 413

Performing Lab

Mayo

LOINC Code(s)

6765-2

Additional Information

Test Definition: 17OHP

Steroid Pathways