Test Directory

Neurofilament

Clone:

2F11 (Spokane)

Application:

In general, the neurofilament (NF) immunostain is useful for detecting the cytoplasmic projections of mature neuronal cells (e.g. axons), either within the CNS or PNS. 

This can be applied to the differential diagnosis of neurofibroma vs schwannoma (neurofibromas are more likely to have intratumoral axons). It can also be used to detect CNS infarction (loss of NF staining).  In conjunction with luxol-fast blue, it is also great for diagnosing demyelinating disease within CNS specimens (MS plaques will show preserved NF staining with loss of LFB staining.

Of note, relatively immature/primitive neuronal cells (neuroblastoma or medulloblastoma) show significantly lower levels of staining.

Specimen Type:

Formalin-fixed, paraffin-embedded tissue blocks (FFPE). If tissue blocks are not available, unstained slides are acceptable (please submit 1–2 slides per antibody to be tested, cuts must be 4 microns thick).

Collection Procedure:

Tissue removed during a surgical procedure and placed in 10% NBF soon after removal from the patient. Appropriate tissue for requested staining.

Required Information:

Complete the Incyte Diagnostics requisition with the patient's demographics and relevant clinical history.

Specimen Stability:

Ambient: Indefinite

Unacceptable Specimen:

Slides or paraffin blocks with no tissue remaining. Samples fixed in fixatives other than 10% neutral buffered formalin. Paraffin blocks that have been overheated or frozen.

Department:

Histology

Method:

IHC, Ventana Ultra Stainer

Turnaround:

12 business days from receipt

Performed:

Monday–Saturday

Retention:

Blocks: 10 years
Slides: 10 years

CPT Code:*

88341
88342

*The listed CPT codes are for reference use only. Final coding is based on CPT guidelines and individual circumstances.

1-888-814-6277

©2019 Incyte Diagnostics. 13103 E Mansfield Ave, Spokane Valley, Washington 99216 USA. 509.892.2700. Website Design by Klündt | Hosmer. All rights reserved.