CMV Qualitative by PCR, Tissue/Cells

Cytomegalovirus by PCR-Tissue or Cells, PCR-CMV-Tissue or Cells, UOW722

The detection of CMV by real-time PCR amplification. PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Detection of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is 20 IU/mL (1.3 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).

Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube.

Bone Marrow: 5 mL blood in EDTA (Lavender Top) tube, No Heparin aspirate in syringe.

Swab: In sterile tube/universal transport media (UTM).

Cellular fluid: 1 mL in sterile tube

Tissue Biopsy, Paraffin Tissue, Bone Marrow, Swab or Cellular fluid (e.g. BAL or Washing)

Bone Marrow: 5 mL bone marrow in EDTA (Lavender Top) tube to Laboratory at room temperature within 24 hour of collection.

ONLY COLLECT MONDAY-THURSDAY AND MUST BE RECEIVED IN THE LAB WITHIN 24 HOURS OF COLLECTION TO BE TRANSPORTED TO UW TO MEET THE 48 HOUR REQUIREMENT

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

0.5 mL

Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder.

Tissue/Cellular fluid/Swab: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.

Virology, Eastlake (EVIR)

Real-time Polymerase Chain Reaction.

Wednesday. Results following Thursday.

CMVQLS      -    CMV Qual Specimen Description
CMVQLR     -    CMV Qual Result
CMVQLI       -    CMV Qual Interpretation

87496

No

UW

4998-1