Test Directory

Kappa ISH

Clone:

Kappa DNP Probe Cocktail

Synonym(s):

Kappa by in situ hybridization

Application:

Kappa DNP ISH probes bind to kappa light chain mRNA in formalin fixed, paraffin embedded tissues. In combination with Lambda DNA ISH testing, this assay assists in the demonstration of monoclonal plasma cells and determining immunoglobulin.

Specimen Type:

Formalin-fixed, paraffin-embedded tissue blocks (FFPE). If tissue blocks are not available, unstained slides are acceptable (please submit 1-2 slides per antibody to be tested, cuts must be 4 microns thick).

Collection Procedure:

Tissue removed during a surgical procedure and placed in 10% NBF soon after removal from the patient. Appropriate tissue for requested staining.

Required Information:

Complete the Incyte Diagnostics requisition with the patient's demographics and relevant clinical history.

Specimen Stability:

Ambient: Indefinite

Unacceptable Specimen:

Slides or paraffin blocks with no tissue remaining. Samples fixed in fixatives other than 10% neutral buffered formalin. Paraffin blocks that have been overheated or frozen.

Department:

Histology

Method:

Ventana, RNA probe, CISH

Turnaround:

12 business days from receipt

Performed:

Monday–Saturday

Retention:

Blocks: 10 years
Slides: 10 years

CPT Code:*

88364
88365

*The listed CPT codes are for reference use only. Final coding is based on CPT guidelines and individual circumstances.

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