Laboratory Services

Tacrolimus, Blood

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Updated Test Information:

Test Description
Tacrolimus, Blood
Synonym(s)

FK506; Prograf

Test ID
TAKRO
General Information

Useful for:



  • Monitoring whole blood tacrolimus concentration during therapy, particularly in individuals coadministered CYP3A4 substrates, inhibitors, or inducers

  • Adjusting dose to optimize immunosuppression while minimizing toxicity

  • Evaluating patient compliance

Specimen Type

EDTA Whole Blood

Specimen Requirements

Lavender top (EDTA)

Specimen Collection / Processing Instructions

  • Draw blood immediately before a schedule dose

  • Do not centrifuge

  • Send specimen in original tube

Stability

  • Refrigerated (preferred): 14 days

  • Ambient: 14 days

  • Frozen: 14 days

Limitations

  • The recommended therapeutic range applies to trough specimens drawn immediately before a dose

  • Blood drawn at other times will yield higher results

Methodology

High-Performance Liquid Chromatography/Tandem Mass Spectrometry (HPLC-MS/MS)

Estimated TAT

1-3 days

Testing Schedule

Monday-Sunday

Retention

14 days

CPT Code(s)

80197

Additional Information
Minimum Sample Volume

1 mL

Reference Range

5.0-15.0 ng/mL (Trough)



Target steady-state trough concentrations vary depending on the type of transplant, concomitant
immunosuppression, clinical/institutional protocols, and time post-transplant. Results should be interpreted in
conjunction with this clinical information and any physical signs/symptoms of rejection/toxicity.

Performing Lab

Mayo

LOINC Code(s)

77348-1