Useful for detection of spinal fluid in body fluids, such as ear or nasal fluid
Preferred: Sterile container, syringe, test tube, or microtube
Acceptable: Plain cotton swab or gauze
Specimen Collection / Processing Instructions
If submitting a syringe, remove needle. Add cap to end of syringe
If direct collection is not feasible, specimen may be collected using a plain cotton swab or gauze
If gauze is used to collect specimen, circle area on the gauze where specimen was collected
Place cotton swab or gauze in as small a container as possible (eg, plain test tube or collection container)
Do not collect specimen with a culture swab
Do not add any liquid to the swab or gauze.
Additional Processing Details
If specimens are collected from multiple sites on the body (ie, left and right), each specimen must be sent under a separate order.
Samples collected from above the shoulders risk salivary contamination, which can degrade the beta-2 transferrin protein. These samples should be frozen immediately following collection and kept frozen until testing is performed
Although results may be obtainable on smaller specimens (perhaps as little as 0.05 mL, depending on the protein concentrations and percentage of spinal fluid in the specimen), reliable results are best obtained with an adequate specimen volume.
Indicate specimen source
Include whether the source is from the right or left side of the body, if applicable
Frozen (preferred): Not Given
Ambient: Not Given
Refrigerated: Not Given
Unacceptable Specimen Conditions
Samples collected with additives such as microbiology media (eg, Stuart or Amies liquid medium) or TransFix/EDTA (used for analyses in flow cytometry) yield uninterpretable results and will be rejected.
Beta-2 transferrin is also found in aqueous humor and in serum of patients with rare metabolic glycoprotein disorders or genetic variants of transferrin
Samples stored in storage tubes with TransFix/EDTA (used for analyses in flow cytometry) are not acceptable. The contact of cerebrospinal fluid (CSF) with the stabilization solution contained in these tubes changes the migration and the result is not interpretable